Isolation of Bursaphelenchus sinensis (Nematoda: Parasitaphelenchidae) from dead Japanese black pine, Pinus thunbergii Parl. in Japan
Keywords:
Bursaphelenchus sinensis, Japan, molecular profiles, morphology, taxonomyAbstract
Bursaphelenchus sinensis was isolated from a dead Japanese black pine, Pinus thunbergii in Japan. The Japanese population of B. sinensis was compared with the original descriptions of a B. sinensis population from China and of the close relative, B. aberrans from China. The molecular profiles of B. sinensis were determined by DNA sequencing and ITSRFLP analyses, and were compared with those of the other species in the genus. By morphological observations, several additional characters, such as a three-celled structure at the junction of the uterus and post-uterine sac, which has been reported in several Bursaphelenchus species belonging to various phylogenetic groups, were found in B. sinensis. The phylogenetic analysis of the small subunit of ribosomal DNA indicated that B. sinensis is included in an inner clade of the genus Bursaphelenchus; however, its precise phylogenetic position within the genus was not defined clearly.Downloads
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KANZAKI(12), K., & FUTAI, K. (2010). Isolation of Bursaphelenchus sinensis (Nematoda: Parasitaphelenchidae) from dead Japanese black pine, Pinus thunbergii Parl. in Japan. Journal of Nematode Morphology and Systematics, 9(2), J. Nematode Morphol. Syst., 9 (2): 129–136 (2006, publ. 2007). Retrieved from https://revistaselectronicas.ujaen.es/index.php/jnms/article/view/165
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